Agricultural Bacteriology

Cover Agricultural Bacteriology
Agricultural Bacteriology
J E Joseph Eames Greaves
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At this time Winogradsky made a decided improvement in the separation of the nitrifying organism from solutions by use of the Kuhne gelatin silica medium. The nutrient basis of this medium as used by Winogradsky was composed of ammonium sulphate, 0. 41 grams; magnesium sulphate, 0. 05 grams; potassium phosphate, 0. 1 gram; sodium carbonate, 0. 6-0. 9 gram; calcium chlorid, a trace; and water, 100 c. C.
The inoculation of the plates took place either by mixing the inoculating material with the a
...bove solution before the addition of gelatinous silica, or it was made as a streak or smear culture on the already hardened material. In this way the nitrifying organisms developed distinct colonies from which pure cultures were obtained.
The investigations of Winogradsky and simultaneously of War- ington showed the following: (1) That in the soil the nitrifying process was effected by two distinct but closely related organisms, the one converting ammonia into nitrous acid and nitrite and the other changing the nitrites into nitrates.


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