Laboratory Methods of the United States Army

Cover Laboratory Methods of the United States Army
Laboratory Methods of the United States Army
United States Surgeon Generals Office Division
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(6) Incise the nodule with a sterile scalpel.
(c) Evert the edges of the incision with thumb and middle finger, introduce a sterile swab into the center of the lesion, and, exercising care that it does not touch the skin edge of the wound, rotate it gently so as to thoroughly impregnate it with the contents of the lesion.
(d) Remove the cotton plug from the culture tube, flame the neck of the tube and smear the material on the swab over the surface of the culture medium.
(e) Carefully replace t
...he cotton plug in the culture tube, return the swab to the tube in which it was received and forward both tubes to the laboratory.
(/) The presence of B. Mallei in material from open lesions, nasal discharge and sputum (pulmonary lesions in man) can be determined by guinea-pig inoculation (Straus reaction). Collect discharge and forward in sealed container; if neces- sary, add a small amount of saline to prevent drying.
(g) The tubes or containers should be plainly labelled with name (human case) or name or identification mark of animal, the nature of the material, the examination requested, to- gether with the name, rank and station of the veterinarian to whom the reports of the examination are to be forwarded.


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