Vertebrate Histology : a Guide for Course 2, Department of Microscopy, Histology And Embryology. Cornell University And the New York State Veterinary College

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73. Amyelinic nerve fibers. Ox. Isolated fibers from the splenic nerve. Assigned for study. Dissociated in -^X osmic acid (§ 17) ; teased apart with needles.
Both myelinic and amyelinic fibers are present, and care must be exercised in distinguishing between delicate myelinic fibers and the amyelinic ones. When a good place is found, study the amye- linic fibers, noting, as compared with the myelinic nerve-fiber, the absence of the myelinic sheath and the greater number of nerve nuclei. Compare
... the myelinic fibers in this preparation with those in 72. The myelin of the sheath is retained and blackened in 73 ; in 72 it has been dissolved out, PERIPHERAI, NERVES.
74. Sciatic nerve. Cat. Transection ; chrome-oxalic (§ 32) ; paraflBn ; sections fi. Stain with hematoxylin 15 minutes, picrofuchsin ^ minute. Mount in balsam.
Study this preparation for (a) the structure of a nerve trunk, noting its component funiculi or bundles, surrounded by connective tissue sheaths, the perineurium, and bound together by the epi- neurium, while within the funiculi, the individual nerve fibers with the endoneurium ; {b) the structure of myelinic nerve fibers in tran- section, showing the central axis-cylinder, surrounded by the mye- linic sheath (the myelin dissolved out).


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