A Biochemical Study of Nitrogen in Certain Legumes

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We assume that the final substance produced is protein, which is then in part assimilated by the plants with the help of fermentation." Any light which may be throwji on this question will be of great value toward its final solution.
PART I STUDIES TO DETERMINE THRU WHICH ORGAN LEGUMES OBTAIN ATMOSPHERIC NITROGEN For a long time it was believed that the nitrogen fixed by legume bacteria and assimilated by the plant was obtained thru the leaves, and even now many hold to this belief. Frank and O
...tto- in 1890 obtained analytical results which seemed to them to be proof of this theory.
They believed that the bacteria were only incidentally connected with the process, acting perhaps as stimuli.
The first experiment resulting in data of a contradictory nature was made by KossoAvitsch^ in 1891, but the results of this investigation were not generally accepted. Nobbe and Hiltner^ in 1899 added fur- ther evidence to the existing knowledge, but their conclusions, drawn from physiological differences, have not been substantiated by chemi- cal data, which seem more reliable than those of a physiological nature.


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