A Critical Investigation And An Application of the Rat Growth Method for the Study of Vitamin B

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54 67 83 96 111 132 133 145 151 97 *3S gms.: =average weight at death, the average age being 67 days. TABLE XVn. (Results of Tables XV and XVI combined.) No. ,,.„ ,,.„ Age in weeks T T <. . 4 5 6 7 8 9 10 11 12 % Treatment . . , . Total of Diet gain for rats Average weight in grams 8 weeks 13 94 ' 48 53 55 51 45 35* 15 100 25 Unheated 45 64 85 99 110 1,18 123 129 130 84 IS 100 25 Heated dry 6 hrs. 45 62 83 98 106 117 124 130 134 88 IS 100 25 Heated dry 24.hrs. 46 60 77 92 102 112 119 125 132 87 15 100 25 Heated dry 48 hrs. 46 59 76 89 98 113 115 125 128 82 * 35 gms.=average weight at death, 64 days being the average age. The conclusions to be drawn from these regarding the heat destruction of vitamin B in the dry state, confirm those of the previous experiment in which the heated milk was fed separately. The average gain in weight for eight weeks on a control diet containing unheated milk and on the diets con- taining the milk heated for 6, 24 and 48 hours is the same. There is evidently no destruction of the vitamin by this treat- ment or the amount of destruction is too small to be detected by either of the methods employed in the present work.

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