A Laboratory Guide in Bacteriology, for the Use of Students, Teachers And Practitioners

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Routine study. — Study carefully the reaction on milk, and test for indol. Preserve glucose-agar-cultures for two weeks.
Digitized by VjOOQIC 86 LABORATORY GUIDE IN BACTERIOLOGY 2. Special study A. — Inoculate fermentation-tubes in the same manner as in the two preceding groups. Ob- serve the absence of gas-formation but note growth in both arms. Compare the results with those of the colon and intermediate groups.
3. Special study B. — The staining of flagella. — Read carefully in the textbook
...the methods' of staining flagella.
To demonstrate the presence of flagella on B. typhosus, the following method will give good results (Loeffler's method) : a) Prepare the mordant. (What is a mordant ? See the textbook.) Tannic acid (25^ aqueous solution) 10 parts Ferrous sulphate (saturated aqueous solution) . . . 5 parts Fuchsin (saturated alcoholic solution) 1 part b) Prepare a number (six) of cover-slips by carefully flaming them, and place them side by side on a piece of filter-paper.


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