A Review of the Existing Methods for Cultivating Anaerobic Bacteria

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Introduce hydrogen into each tube for about five minutes, then place the apparatus in the incubator. In addition, this writer pours on top of the liquid paraffin a layer of the following mixture : Paraffin, solid, 50 parts ; cera flava, 20 parts ; vaseline, 30 parts. According to Vottelier this makes an absolutely air-tight seal. The author recommends the use of large test tubes for this purpose, as in case of small test tubes the slanted agar slides down easily. The agar must be well cooked be...fore slanting. Before inoculation the condensation water is carefully poured off and a loose, sterile cotton plug is inserted ; an occasional infection is made harmless by the pyrogallic acid.
\T Fig. 54.
Digitized by Microsoft® [Reprinted from the JOURNAL OF APPLIED MICROSCOPY AND LABORATORY METHODS, Rochester, N. Y., VoL V, No. 5.] Page 1811 Upon concluding this review the author's attention was drawn to a few appli- ances and methods not described herein. Their inventors are: Zettnow (1894), Migula (1895), Klein (1898), Epstein (1898), Bombicci (^mi), and Turro (1902).


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