A Study of the Action of Ammonia On Thymol

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Violet (bluish) 19. Aniline 55 The common acids and alkalis proved of no better service.
Concentrated sulphuric acid, however, forms a sulphonate which is soluble in water. To some of the pigment, then, some cone, sulphuric acid was added, and the solution allowed to stand for several hours. This was now poured into a large excess of water, and a small quantity of a greenish-colored pre- cipitate was obtained.
As thymol is volatile with steam, this was thought of as a possible means of separati
...on, and it proved very good. When the pigment was steam distilled, thymol came over, leaving the pure pigment behind. The steam distillation was continued till the distillate no longer gave a sulphuric acid glacial acetic acid test (a "control" was used, for even with a "control" a slight pink is obtained). The solution in the distilling flask was now extracted with ether (sodium chloride was for some time used to first saturate the solution, but this contaminated the ether extract, and was eventually discarded), and the ether evaporated.

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