A Study of the Physiological Relations of Sclerotinia Cinerea (Bon) Schröter

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omnivorous Van Hall, and that it may possibly be identical also with Potter's B- destrudans. By many tests Jones has shown that this organism secretes an enzyme which causes the disintegration of the host cells by dissolving the middle lamella, which, according to the majority of investigators, is composed of salts of pectic acid.
This author has further isolated from pure cultures of the or- ganism an extra-cellular enzyme, which he designated pectinase, that destroys the middle lamella of the
... cells just as does the growing organism. Jones, therefore, considers this enzyme responsible for the disintegrating action of the bacillus.
In my own work I shall adopt the nomenclature used by Jones (29, 30) and Euler (21), namely, employing pectinase as the term to designate the enzyme inducing coagulation of a pectin solu- tion and also the hydrolysis of calcium pectate, or pectinate.
Methods. — In order to determine the effect of the fungus on the middle lamella I have used two methods, (1) a microscopic study of the effect of the fungus on the host cells, and (2) a study of the effect of the organism on the substances (isolated from the host) which are commonly reported to be constituents of the middle lamella.


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