A Text-Book of the Physiological Chemistry of the Animal Body, Including An Account of the Chemical Changes Occurring in Disease; V.2

Cover A Text-Book of the Physiological Chemistry of the Animal Body, Including An Account of the Chemical Changes Occurring in Disease; V.2
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" Take 2 cc of extract 6 + 8 c.c of dilute HCl (0-2 p. c). Add to this and to each of the various pepsin solutions made with extracts a, 1 c.c. of carmine-stained and already swollen fibrin (which has been previously measured and placed aside in watch glasses), note which of the (a) mixtures digests at the same rate as (h) ; if, for example, the (a) mixture in test tube (3) digests at the same rate as (h), then the original (a) extract contains eight times as much pepsin as (h).
" 2. In compari
...ng the amount of pepsin which can be extracted from equal weights of ditferent stomachs, or of different parts of any one stomach, the mucous membi'ane should be rapidly washed with salt solution, and the salt solution then sopped up with blotting paper. The stomach should, then, be spread out on glass and the muscular coat removed; the mucous membrane should then be dried first at about 25° C. and then over sulphuric acid. Weighed portions of mucous membrane should then be cut into small pieces and treated with a 2 p.

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