Alcoholic Fermentation

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The action of toluene has hitherto been most completely studied, and this substance is an extremely suitable one for the purpose since it has practically no action whatever on fermentation by yeast-juice. The experiments of Buchner have, in fact, shown that the normal rate of fermentation and the total fermentation produced, are almost unaffected by the presence of toluene even in the proportion of I c. C. To 20 c. C. Of yeast-juice. What then is the effect of toluene on the living yeast- cell?... When toluene in large excess is agitated with a fermenting mixture of yeast and sugar, the rate of fermentation falls rapidly at first and then more slowly until a relatively constant rate is attained which gradually decreases in a similar manner to the rate of fermenta- tion by yeast-juice. Thus at air temperature (16) 10 grams of THE MECHANISM OF FERMENTATION 125 yeast suspended in 50 c. C. Of 6 per cent, glucose solution gave the following results when agitated with toluene : Time after Addition of Toluene Minutes.

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