An Application of Polarized Light to Resolution With the Compound Microscope

Cover An Application of Polarized Light to Resolution With the Compound Microscope
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Now without disturbing the arrangement of the lamp or the prisms, the mirror should be shifted until a beam from the same lamp is reflected from the mirror into the prism first placed, as is clearly shown in Plate VII, and which will emerge from the bottom edge of the back lens.
IMAGE SHOWING STRUCTURE RESOLVED INTO " DOTS ".
The proper appearance of the back lens at this time is shown in Plate VIII, fig. 8. The reflected beam with its accompanying diffracted beam will form an image show- ing t
...ransverse striae, which will blend with the im.age already formed showing longitudinal striae. However the Image showing transverse striae probably will be brighter than the other.
The eyepiece should then be replaced and the mir- ror shifted slightly until the intensities of the two beams are balanced so that the resulting image shows the surface of the diatom covered with clearly defined dots as shown in Plate VIII. A little practice may be required 19 to effect this balance of intensity, but if a sharp clear image showing longitudinal lines is formed at first, a good image showing dots should be readily obtained, its perfection depending upon the quality of the objective employed.


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