Bacteriology a Manual for Students And Practitioners
Bacteriology a Manual for Students And Practitioners
Frederick Carl Zapffe
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The immunization must be carried on judiciously. Care must be taken not to exhaust the tolerance of the animal, as a condition of hy perse nsitivity may be produced to which the animal usually succumbs. There is, in short, a limit to the tolerance of the animal, and this limit must not be passed. The serum of highly immunized horses is most suitable for therapeutic purposes. IMMUNIZATION. 129 A lower serum standard is sufficient for protective purposes. Larger amounts of the antitoxic serum mus...t be used to pro- duce a cure than for immunization against infection. When the horse possesses the desired tolerance, the jugular vein is laid bare under antiseptic precautions and a sterile trocar thrust into the vein. The blood is collected in sterile bottles, and after it has coagulated it is placed on ice for a few days, when the clear supernatant serum is pipetted off with sterile pipettes. This serum contains the antitoxic sub- stance, and is known as the "antitoxin. " The serum is then standardized and placed in glass containers, which are steril- ized and then hermetically sealed.
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