Botanical Micro Chemistry An Introduction to the Study of Vegetable Histology

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Cilia and the bodies of the cells are sharply differentiated by this method. 2 Koch has later recommended coloring with haem- atoxylin. Staff-shaped bacteria, however, do not color by this substance, according to him. 3 I have however used the haematoxylin tincture with suc- cess even on certain staff-shaped bacteria. After rinsing, my preparations are preserved dry.
Histologie, p. 103. Robin: Microscope, 1877, p. 250. Bachmann: Dauer- praparate, p. 28. Schmitz : Niederrhein. Gesellsch. , Nov.
...1879.
1 Johow: Zellkerne d. Hoheren Monocotylen. , Diss. , Bonn, 1880, p. 9, note.
2 Koch : Conserviren und Photographiren der Bacterien. Cohn's Beitr. Z. Biol. D. Pfl. , II. , p. 421.
3 Wundinfectionskrankheiten, 1878, p. 30.
COLORING AGENTS. 57 NIGRO&N.
[Errera recommends x this tar derivative for staining the nucleus. It is soluble in water, but insoluble in alcohol and ether. After remaining a short time in a solution of nigrosin, the section is transferred to water, where it remains until no more of the color is removed, when it may be mounted in glycerine or glycerine jelly, or trans- ferred to alcohol, and afterward cleared in oil of cloves and mounted in balsam.


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