Cell-Mediated And Humoral Immune Responses to Dirofilaria Immitis in Experimentally-Infected Dogs.
Cell-Mediated And Humoral Immune Responses to Dirofilaria Immitis in Experimentally-Infected Dogs.
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Those fractions within the initial, principal protein peak were combined, lyophil ized, and resolubilized with 5 ml of 0.005M sodium acetate (pH 4.6).
The sodium acetate solubilized preparation was layered onto a 5 carboxymethyl cellulose column equilibrated with sodium acetate (pH 4.6) and eluted at a flow rate of 20 ml/hour. The eluate was monitored and collected as above and after each protein fraction was completely eluted, the column was eluted with a buffer of increasing molarity or pH. T...he eluants used in succession were 0.005M sodium acetate (pH 4.6), 0.05M phosphate buffer (pH 6), 0.1M sodium chloride (pH 7), 0.2M sodium chloride (pH 7), 0.4M sodium chloride (pH 7), 0.1T hsC0 Chromatographic Column, ISC0, Lincoln, MB 2 Sephadex G-100, Pharmacia Fine Chemicals, Piscataway, NJ 3 UA-5 Absorbance Monitor, ISC0, Lincoln, NB 4 Model 328 Fraction Collector, ISC0, Lincoln, NB 5 CM-cellulose, Pharmacia Fine Chemicals, Piscataway, NJ sodium hydroxide (pH 7). The fractions eluted with each of these eluants were collected separately; previous to use in the indirect hemagglutination (IHA) assay the fractions eluted with 0.2M and 0.4M sodium chloride were combined and dialyzed against PBS (pH 7.2) for 48 hours at 4°C.
What to read after Cell-Mediated And Humoral Immune Responses to Dirofilaria Immitis in Experimentally-Infected Dogs.?
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To quickly assess the difficulty of the text, read a short excerpt:
Those fractions within the initial, principal protein peak were combined, lyophil ized, and resolubilized with 5 ml of 0.005M sodium acetate (pH 4.6).
The sodium acetate solubilized preparation was layered onto a 5 carboxymethyl cellulose column equilibrated with sodium acetate (pH 4.6) and eluted at a flow rate of 20 ml/hour. The eluate was monitored and collected as above and after each protein fraction was completely eluted, the column was eluted with a buffer of increasing molarity or pH. T...he eluants used in succession were 0.005M sodium acetate (pH 4.6), 0.05M phosphate buffer (pH 6), 0.1M sodium chloride (pH 7), 0.2M sodium chloride (pH 7), 0.4M sodium chloride (pH 7), 0.1T hsC0 Chromatographic Column, ISC0, Lincoln, MB 2 Sephadex G-100, Pharmacia Fine Chemicals, Piscataway, NJ 3 UA-5 Absorbance Monitor, ISC0, Lincoln, NB 4 Model 328 Fraction Collector, ISC0, Lincoln, NB 5 CM-cellulose, Pharmacia Fine Chemicals, Piscataway, NJ sodium hydroxide (pH 7). The fractions eluted with each of these eluants were collected separately; previous to use in the indirect hemagglutination (IHA) assay the fractions eluted with 0.2M and 0.4M sodium chloride were combined and dialyzed against PBS (pH 7.2) for 48 hours at 4°C.
What to read after Cell-Mediated And Humoral Immune Responses to Dirofilaria Immitis in Experimentally-Infected Dogs.?
You can find similar books in the "Read Also" column, or choose other free books by Grieve, Robert Burton, 1951- to read onlineMoreLess
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