Electrokinetic Properties of Calcium Oxalate Monohydrate

Cover Electrokinetic Properties of Calcium Oxalate Monohydrate
Electrokinetic Properties of Calcium Oxalate Monohydrate
Curreri, Peter Angelo
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1 1 \ 33 \\ w *- \\ IN 10 — CO o CO o LlI o o o o ro O C\J OV/ 3 77 We can obtain values for the percent coverage by estimating the projected area of adsorbed serum albumin at various calcium concentrations and then comparing this to the total calcium oxalate surface area available. We did this in the following manner. Values for AC for a series of calcium concentrations were obtained from Fig. 13 (some values were estimated using the line drawn through the data points). The value of 0.027 g/L... was used for A; values for AC were calculated from Eq. [7].
The surface area of the calcium oxalate used in these experiments as measured by nitrogen gas adsorption is about 3.0 m"/g. The molecular weight and density used for serum albumin were 69,000 g/M and 0.73 g/cm re- spectively. Serum albumin was assumed to be spherical in shape. This yields a projected cross -sectional area for adsorbed protein on the surface, Ap , of about 2.3 x 10 A /g. The effective area covered by serum albumin for each solution in Angstroms squared per liter is A = AC x Ap [8 *This value for surface area gives a more conservative estimate of crowding than the lower surface area which was estimated for the powder in the adsorption study using the optical microscope.


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