Elements of Bacteriology for Practitioners And Students With Especial Reference

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See Appendix. ] TE.
METHODS OF STAINING BLOOD 277 the blood is obtained by pricking it. The first drop having been wiped away with a sterilised platinum needle, the second is taken off with a disinfected cover-glass, spread out by rubbing with another cover-glass (also disinfected), dried, and heated to 120 0. Approximately. Disinfection of the cover-glasses is best done in corrosive sublimate, from which they are transferred to alcohol and ether with a sterilised forceps, and then taken out an
...d rapidly dried in the air. The basic aniline dyes, commonly used for bacteriological examination, are employed in aqueous solu- tions, with the occasional addition of alcohol, glycerine, or acetic acid to the fluid. The stains are allowed to act for some minutes and then rinsed off with distilled water ; the preparation is dried and Canada balsam applied to it, or if it is not wished to keep the specimen for any length of time, it is mounted temporarily in oil of cloves, origanum, or cedar. Loffler's alkaline solution of methyl blue [pp.

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