Histological Technique

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Formulas (a) and (b) may be used with sections and in the same way as eosin.
§ 107. Picric Acid. A counter-stain useful after carmine or hema- toxylin.
Employ a 1-5% alcoholic solution, or simply add to the alcohol used in dehydration. It washes out the hematoxylin and is useful as a differ- entiator of this stain, with which it is well to overstain somewhat if it is desired to counter-stain with picric acid.
§ 108. Orange G. An excellent acid stain that may be employed as counter-stain and dif
...ferentiator after hematoxylin, gentian violet, etc.
Employ a 2 to 4% aqueous solution which may with advantage be slightly acid (Hcl.) ; in the last event, sections should be rinsed well be- fore the mounting. It may with good effect be combined with eosin, eryth- rosin or fuchsin acid.
§ 109. Fuchsin Acid (Rubin S., Magenta S.). Like Congo red, an indi- cator, red in acid of neutral solutions, bleached by alkali. It is a valuable plasma stain, but requires care in its use because of its sensitiveness to alkali.


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