Hog Cholera; Its History, Nature, And Treatment

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(212° F.)..
70° C. (158° F.)...
5S°C. (138° F.)...
54.5° C. (130° F.).
49° C. (120° F.)...
Immediately.
4 minutes.
15 minutes.
1 hour.
Not in 2 hours.
Bacteria do not so readily succumb to heat when dried and then ex- posed to dry hot air. It was found that dry heat at 80° O. (176° F.) is sufficient to destroy the bacteria when exposed in a dry state for fifteen minutes.
In these experiments bacteria from cultures were rubbed on the inner side of plugged and sterilized test tubes. Gelatine was
...added after they had been exposed to the heat and roll cultures made. Absence of growth indicated the death of the contained bacteria.
Resistance to freezing is well illustrated in the experiments reported in the section on the vitality of hog cholera bacilli in the soil during winter.
Vitality of hog cholera bacteria in ordinary water. — The hardiness of this microbe is well illustrated by its capacity for multiplication in or- dinary drinking water. To determine this, the following experiment was made : September 8, a culture tube containing very clear Potomac drinking water* which had been sterilized several weeks previous by a tem- perature above 110° C, was inoculated with a platinum loop from a pure liquid culture of the bacillus.


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