Laboratory Guide for the Bacteriologist ..

Cover Laboratory Guide for the Bacteriologist ..
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The boiling is accomplished by holding the cover-glass in the lower portion of the Bunsen flame until the staining fluid steams; then slowly raise the glass to the top of the flame and the fluid will boil; immediately lower again and repeat.
5. Wash in water and partially dry the cover-glass with filter-paper.
6. Flood the glass with Gabbet's solution,^ from one to three minutes, according to the thickness of the prepa- ration and to the intensity of the fuchsin stain.
7. Wash in water; place t
...he cover-slip ("butter" side down) on the slide ; absorb the water with filter-paper, etc., and examine ; or, to obtain a better contrast, stain (this is 110 longer the method of Ziehl-Gabbet).
(b) 7. After 6, wash in water.
8. Stain as usual with 2 per cent, methylene-blue (See 1, foot-note 2, a).
9. Wash in water.
10. Mount, etc., or, after 4: ^ (c) 5. Wash in water.
6. Decolorize in 25 per cent, nitric acid, from ten to thirty seconds.
7. Complete decolorization in alcohol.
S. Wash in water.
9.


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