Manual for the Physiological Chemical Laboratory. for the Use of Medical Students

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This change may take place in the body and the urine voided under such circumstances will be alkaline due to volatile alkali, will probably have an odor of ammonia and give white fumes of ammonium chloride with a drop of hydrochloric acid, if held over it on a glass rod.
Experiment. Heat a little urea in a dry test tube.
It melts, decomposes, and gives off ammonia (NH 3 ), and leaves finally a white mass, which if dissolved in water and treated with a drop of copper sulphate (CuS0 4 ) and an ex
...cess of caustic soda, gives a beauti- ful reddish-violet liquid. (Biuret reaction.) Urea is decomposed by an alkaline solution of sodium hypochlorite, or, better, hypobromite into nitrogen, car- bon dioxide, and water, according to the following equation : CH 4 N 2 0+3NaBrO = N 2 +C0 3 +2H 3 0+3NaBr.
Experiment. Place a little urea solution in a test tube and add some sodium hypobromite. A violent decomposition takes place with the rapid evolution of gas. Let the gas bubbles subside and apply a lighted match to the gas in the test tube.


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