Manual of Vital Function Testing Methods And Their Interpretation
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Determination of the Total Non-Protein Nitrogen. "To determine the non-protein nitrogen of the blood 5 c. C. Of the alcoholic filtrate is transferred to a large Jena test tube. One drop of sulphuric acid, one of kerosene and a pebble are added and the methyl alcohol is driven off by immersing the test tube in a beaker of boiling water for five to ten minutes. When the alcohol is removed 1 c. C. Of concentrated sulphuric acid, a gram of potassium sulphate, and a drop of copper sulphate solution ...are added and the mixture is boiled, cooled and diluted.
"From this digestion mixture the ammonia is removed in the usual manner. It is, however, not collected di- rectly in a measuring flask (as in urine analysis) but in a second large test tube previously charged with 1 c. C. Of acid added to 3 c. C. Of water. The reason for this variation is that 0. 4 to 0. 5 c. C. Of blood con- tains only 0. 1 to 0. 2 mgm. Of non-protein nitrogen. The final Nesslerizcd solution cannot be diluted to 100 c. C.
What to read after Manual of Vital Function Testing Methods And Their Interpretation?
You can find similar books in the "Read Also" column, or choose other free books by Wilfred Mason Barton to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
Determination of the Total Non-Protein Nitrogen. "To determine the non-protein nitrogen of the blood 5 c. C. Of the alcoholic filtrate is transferred to a large Jena test tube. One drop of sulphuric acid, one of kerosene and a pebble are added and the methyl alcohol is driven off by immersing the test tube in a beaker of boiling water for five to ten minutes. When the alcohol is removed 1 c. C. Of concentrated sulphuric acid, a gram of potassium sulphate, and a drop of copper sulphate solution ...are added and the mixture is boiled, cooled and diluted.
"From this digestion mixture the ammonia is removed in the usual manner. It is, however, not collected di- rectly in a measuring flask (as in urine analysis) but in a second large test tube previously charged with 1 c. C. Of acid added to 3 c. C. Of water. The reason for this variation is that 0. 4 to 0. 5 c. C. Of blood con- tains only 0. 1 to 0. 2 mgm. Of non-protein nitrogen. The final Nesslerizcd solution cannot be diluted to 100 c. C.
What to read after Manual of Vital Function Testing Methods And Their Interpretation?
You can find similar books in the "Read Also" column, or choose other free books by Wilfred Mason Barton to read onlineMoreLess
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