Methods in Plant Histology

Cover Methods in Plant Histology
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In the cell of a root-tip a series of small canals, or vacuoles, appears, which is much more definite and extensive than the usual display of vacuoles and which appears before any vacuoles can be recognized in preparations made in the usual way (Fig. 24, B). Being a zoologist, he has called these vacuoles canaliculi.
Bensley's Fixing Agent.
1. Formalin (neutral) 10. C. C.
2. Bichromate of potash 2. 5 g.
3. Corrosive sublimate 5. G.
4. Water 90. C. C.
Dissolve the bichromate of potash in the wat
...er, then add the corrosive sublimate and finally add the formalin. The solution 2, 3, 4 will keep, but the formalin soon becomes acid. Add the formalin to 2, 3, 4 only when needed for use. Obtain the neutral formalin by distilling the ordinary commercial formalin. Proceed as follows: 1. Fix 24 hours.
2. Wash in water, 24 hours.
3. Iodize on the slide.
4. Wash in water, 5 minutes.
5. Copper acetate (neutral) saturated solution in water, 5 to 10 minutes.
6. Wash in water, 1 minute.
7. ^ per cent haematoxylin, 5 to 10 minutes.


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