Microscopy And Bacteriology

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Microscopy And Bacteriology
P Paul Mile Archinard
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The description of this microorganism given by Kitasato is as follows : Actively motile rods, 3 to 5 mikrons in length, and from 0. 5 to 0. 6 mikron in thickness, occurring singly, occasionally in pairs, never forming filaments (Fig. 62).
It stains by all the anilin colors and by Gram's method. It forms spores, which are situated at or near one of the poles, giving a swollen appearance to the bacillus.
Biologic Characters. In the vegetative type it is actively motile, but loses its motion in th
...e spore-bearing form. It can not be cultivated in an atmosphere of oxygen. It is purely anaerobic, and does not grow in an atmosphere of car- bonic acid gas.
It grows best when glucose (1. 5 to 2 per cent. ) or glyc- erin (4 to 5 per cent. ) is added to the culture-medium. It grows in all media. It liquefies gelatin. It grows best at the temperature of the incubator, 37 C. , but does not grow at a temperature below 14 C. In deep-seated punct- ures of gelatin or agar it grows in three or four days, and produces during its growth gas bubbles.


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