Milk

Cover Milk
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The antiseptics used were chloro- form, ether, benzol, and toluol, but preference was given to chloroform as being the most effective. These antiseptics pre- vent bacterial growth, but do not inhibit enzym activity.
232 MILK Harding and Van Slyke, in a study of the quantity of chloro- form required to restrain bacterial growth without materially affecting enzym action, have observed that some of the chloroform is absorbed Ijy the fat and then becomes useless as a restraining agent. The greater
...the percentage of fat in the milk, the larger the amount of chloroform lost. It settles to the bottom with the dis- solved fat. Skimmed milk containing 3 per cent, protein required 0.2 per cent, by volume of chloroform to destroy vegetative forms of bacteria gradually and 0.4 per cent, to destroy them within twenty-four hours. In whole milk of 5 per cent, fat content 1 per cent, chloroform destroyed the vegetative forms gradually, 1.5 per cent, within twenty-four hours, and 2 per cent, in fo.ur hours.

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