Pathological Technique; a Practical Manual for the Pathological Laboratory

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Potato. — Growth occurs, but it is usually invisible.
Dunham's Pepton Solution. — No indol-production — i. e.
no red color appearing in the twenty-four- to forty-eight- hour cultures after the addition of 5 drops of concentrated sulphuric acid, c. p., and i cubic centimeter of a solution of sodium nitrite, i : 10,000.
Motility. — Very marked.
BACTERIOLOGICAL EXAMINATIONS.
143 Flagella (Fig. 45) may be demonstrated by the special methods of staining described elsewhere.
Decolorized by Gram's met
...hod. Does not form spores.
Bouillon, — Clouded, with the formation of some sediment.
The clouding of the medium is not so marked as in the case of the bacillus coli communis. In general, the growth of the typhoid organism is not so vigorous on culture-media as is the growth of the bacillus coli communis.
When to a bouillon culture a small quantity of the blood- FlG. 45. — Bacillus of typhoid fever, from an agar-agar culture six hours old, showing the flagella stained by Loffler's method ; x looo (Frankel and Pfeiffer).


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