Pathological Technique a Practical Manual for Workers in Pathological Histology

Cover Pathological Technique a Practical Manual for Workers in Pathological Histology
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Oleum origani cretici.
7. Xylol balsam.
Neutral Carmine (see page 283). — Neutral carmine is a diffuse stain, and is employed more especially for the central nervous system and for bone.
Filter one or two drops of the solution into 20 c. C. Of dis- tilled water, and leave the sections in the dilute solution over night. It is advisable to place a piece of filter-paper on the bottom of the dish for the sections to rest on, otherwise they may be stained on the upper side only. In double stains wit
...h hematoxylin and carmine the sections should be stained first in the hematoxylin and then thoroughly washed in water for six to twelve hours before they are stained in the carmine. After the carmine they are again to be thoroughly washed in water.
Combination Stains. — Biondi-Heidenhain Stain (see p. 289). — Tissues must be hardened in corrosive sublimate.
1. Stain paraffin sections six to twenty-four hours with the dilute solution.
2. Wash out a little in 90 per cent, alcohol.
3. Dehydrate in absolute alcohol.


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