Pathological Technique: a Practical Manual for Workers in Pathological Histology And Bacteriology, Including Directions for the Performance of Autopsies And for Clinical Diagnosis By Laboratory Methods.

Cover Pathological Technique: a Practical Manual for Workers in Pathological Histology And Bacteriology, Including Directions for the Performance of Autopsies And for Clinical Diagnosis By Laboratory Methods.
Pathological Technique: a Practical Manual for Workers in Pathological Histology And Bacteriology, Including Directions for the Performance of Autopsies And for Clinical Diagnosis By Laboratory Methods.
Frank Burr Mallory
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Counterstain in alum-hematoxylin.
4. Wash thoroughly in water.
5. Mount in glycerin or glycerin jelly.
The staining should always be done in a tightly stoppered bottle, because with any evaporation of the alcohol a precipi- tation of the staining material immediately takes place.
If, after staining with alum-hematoxylin, the sections are put into a i per cent, aqueous solution of acetic acid for three to five minutes, the color of the nuclei is a clearer blue, in better contrast with the red co
...lor of the fat, and the staining is sharper.
25 Digitized by Google 386 PATHOLOGICAL TBCHNIQUE, Herxheimer recommends the following solution as stain- ing much more quickly — two to five minutes.
Absolute alcohol, 70; 10 per cent, solution of sodium hydrate, 20; Water, 10.
Scharlach R. to the point of saturation.
Benda's Stain for Fat Acid CrystalB. — In a solution of copper acetate the crystals of the free fatty acids and of the fatty acid salts of lime stain blue. Benda recommends fixa- tion in Weigert's copper-fluorchrom-acetic-acid-mordant plus 10 per cent, of formalin for two to four days at 37° C.


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