Phosphorus in Beef Animals

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This made a total of 270 cc. of water used for each flask.
The extract was transferred to a 2 -liter measuring flask and then to a glass stoppered bottle of approximately 8 liters capacity. Each Florence flask was rinsed twice with about 12 cc. of water. The 6 liters 1 of the extract were mixed, avoiding aeration, and filtered through a dry filter.
Total Soluble Phosphorus. 500 cc. portions of the extract were meas- ured into 600 cc. beakers and evaporated on the water bath to a volume of about
... 50 cc; with the aid of 15 cc. of sulphuric acid and hot water this was transferred to 500 cc. Kjeldahl flasks. Then about 0.7 gram mercury together with 5 grams of potassium sulphate were added and the solution digested as for nitrogen determinations.
After cooling, the liquid and any residue were washed into a 250 cc.
beaker, slightly diluted with water and neutralized with ammonia. The operation was then completed as under total phosphorus.
Soluble Organic Phosphorus. 2 600 cc. portions of the extract were measured into 1000 cc.


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