Plant Anatomy From the Standpoint of the Development And Functions of the Tissues, And Handbook of Micro-Technic

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This gives a yellow precipitate in sections containing tannins.
Ammonium Vanadate. — This is used as a test for solanin.
The sections are treated with a solution prepared by dissolving I part of ammonium vanadate in 1000 parts of a mixture of 98 parts of concentrated sulphuric acid and 36 parts of water.
If solanin is present, a yellow color appears, which merges into orange, then different shades of red, and finally into violet, and then all color disappears.
ANILINE OIL-^BERLIN BLUE 287 Anili
...ne Oil. — Excellent for dehydrating sections, since it will dissolve about 4 per cent, of water and may be kept dehy- drated by a small piece of solid KOH which is insoluble in it.
The sections may be transferred from the aniline immediately into Canada balsam.
Aniline Sulphate. — Make a saturated aqueous solution.
As a test for lignified membranes mount the sections in the solu- tion and add a drop of sulphuric acid, and a yellow color is given to the lignified membranes.
Or pour sulphuric acid slowly into aniline oil until a pre- cipitate is produced throughout and then add water until the precipitate is dissolved.


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