Practical Histology

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NOTES.
Skin.
Hardening. Mercuric chloride, picric acid and formol, chromic acid, may also be used for hardening skin. Oalleja's stain (p. 311) gives good results. Thin sections of tissue hardened in mercuric chloride may be stained with Mallory's fluid (p. 311).
In imbedding, the tissue should be left but a short time in xylol and in melted paraffin, as in these it is apt to become hard.
For prickle cells, sections of the Malpighian layer parallel to the surface may be cut from skin fixed in os
...mic acid, and moimted in glycerine-jelly.
The horny layer will swell up and the cells show as vesicles with sharply outlined walls, if a piece be cut oflT with a razor, placed in 35 p.c. potassium hydrate for 10 to 15 minutes, and then slightly teased in a drop of water on a slide.
XXVIl] SKIN. SENSORY NERVE ENDINGS 215 Sensory nerve endings.
The endings in mucous membranes, and in tactile corpuscles are best seen in preparations made by the methylene blue injection method. Cp. p. 321.
For other gold chloride methods cp.


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