Scientific American Supplement, No. 385, May 19, 1883

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Prof. Baumgarten has just published in the _Ctbl. F. D. Med. Wiss_. , 25, 1882, the following easy method to detect in the expectorated matter ofphthisical persons the pathogenic tubercle bacilli: Phthisical sputa are dried and made moist with very much diluted potashlye (1 to 2 drops of a 33 per cent. Potash lye in a watch glass ofdistilled water). The tubercle bacilli are then easily recognized with amagnifying power of 400 to 500. By light pressure upon the cover glassthe bacilli are easily
...pressed out of the masses of detritus andsecretion. To prevent, however, the possibility of mistaking thetubercle bacilli for other septic bacteria, or vice versa, the followingprocedure is necessary: After the examination just mentioned, the coverglass is lifted up and the little fluid sticking to its under sideallowed to dry, which is done within one or two minutes. Now the coverglass is drawn two or three times rapidly through a gas flame; onedrop of a diluted (but not too light) common watery aniline solution(splendid for this purpose is the watery extract of a common aniline inkpaper) is placed upon the glass.

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