Souvenir

Cover Souvenir
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After grinding I placed them in alcohol to remove the acid, and then stained them by the best technique known. I failed to discover any " reticulum. " Again, I have taken sections thus prepared and decalcified them under a cover-glass on a slide on the stage of a microscope, carefully watching the process from time to time. Results negative. The fluid used was one-half of one per cent, solution chromic acid.
Again, to avoid all possibility of error in technique, I imbedded sec- tions of freshly
...-ground teeth in celloidin, and decalcified them in a one-half of one per cent, solution of chromic acid, stained and after- wards examined them with a Zeiss one-twelfth horn, oilim. lens, with- 35 out being able to demonstrate any organic tissue. By the last-named process it was not possible for the reticulum to disappear through faulty technique. The celloidin, acting as a perfect imbedding mass, was not affected by the acid in the least degree ; nor did it hinder in the process of staining, for it is well known that it is more permeable to stains than tissue itself.

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