Studies in General Physiology volume 2

Cover Studies in General Physiology volume 2
Studies in General Physiology volume 2
Jacques Loeb
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In 98 c. C. NaCl + 2 c. C. KC1 the eggs reached the sixty-four-cell stage or went even beyond this. It was the same in 96 c. C. NaCl -f 4 c. C. KC1 for almost every egg divided. With more KC1 and less NaCl the results were less favorable. In a former paper we pointed out that the comparative harmlessness of K ions for the phenomena of cell-division is in striking contrast with the harmfulness of the same ions for the phenomena of muscular contraction. We thus see that the following two combinat...ions of two chlorides in solution are the most favorable for development: (1) 90 c. C. Yn MgCl 4- 10 c. C. ^n CaCl 3 ; and (2) 98 c. C. Fn NaCl + 2 c. C. F n KC1.
Three chlorides in solution. Neither with one nor with two chlorides in solution was it possible to obtain swimming blastulse. From the experience with Fundulus 1 I expected that a combination of three metal ions (especially NaCl, with small amounts of KC1 and CaCl 3 ) would allow the eggs of the sea-urchin to complete their development.


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