Studies On the Estimation of Inorganic Phosphorus in Plant And Animal Substances

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26 OHIO EXPERIMENT STATION: TECHNICAL BUL. 8 Wash the coagulum twice with the above wash solution from a wash bottle.
At all times allow the filter to drain well between additions of extract or wash solution. Combine the 10 filtrates, washing out the container of each of the filtrates twice with 5-8 c. c. of distilled water. Make the extract up to 5000 c. c, and mix.
The three methods compared, for the precipitation of inorganic phosphorus in the extracts prepared as above, and for the final es
...ti- mation of the phosphorus, were as specified below : EMMETT AND GRINDLEY NEUTRAL AMMONIUM MOLYBDATE METHOD FOR THE DETERMINATION OF INORGANIC PHOS- PHORUS IN WATER EXTRACTS OF FLESH Measure out the number and volumes of extracts indicated in the schedule on p. 24. Evaporate, with frequent stirring, on the water or steam bath to approximately 20 to 25 c. c. While hot, filter into 300 c. c. beakers, using doubled 11 cm. No. 589 (Blue Ribbon brand) S. and S. papers. Wash beakers, precipi- tates, and filters thoroughly with hot water.

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