Sweet-Clover Seed. Pt. I.--Pollination Studies of Seed Production. Pt. Ii--Structure And Chemical Nature of the Seed Coat And Its Relation to Impermeable Seeds of Sweet Clover

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The greater part of the Ught hne reacted to none of the tests, and its chemical nature was not determined.
Wlien microtome sections of seeds in different stages of develop- ment were treated with various stains, the results were in accord with those obtained with free-hand sections. Thus with safranin the periphery and cones of the Malpighian cells were shghtly stained, while haematoxyhn and methyl blue stained aU the seed coat except the Hght line. The cones and cuticle stained more readily th
...an the cuticularized layer, but neither stained as deeply as the ceU walls below the light line. Methylene blue, methyl violet B, and mauvein stained all above the hght hne, indicating the presence of pectic sub- stances; however, the staining was more prominent in the cones and cuticle.
The difference in reaction of the cones and cuticularized layer to the cellulose and pectose tests probably indicates a difference in density rather than a difference in chemical composition. Since the cuticularized layer separates readily from the cones, there may be a difference in physical properties.


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