The Bacteria in Asiatic Cholera

Cover The Bacteria in Asiatic Cholera
The Bacteria in Asiatic Cholera
Edward Emanuel Klein
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While there can be no question after the detailed experiments of Mr. Watson Cheyne that hydrochloric acid of the strength of 0-2 per cent, kills without fail the comma- bacilli, if allowed to act on them for a few minutes, I do not hesitate to say that with the exception of the spores of bacilli all other bacteria micrococci, bacteria, sporeless bacilli, and vibriones and spirilla are alike killed under similar circumstances by acid : of such strength. 1 And just as a more dilute acid does not ...affect in a few minutes' action the vitality of many other bacteria, so also the comma-bacilli remain unaltered by it. I have mixed choleraic comma- bacilli from a pure culture with a mixture of one part of commercial hydrochloric acid in 1, 000 parts of water for from ten to thirty minutes, and on inoculating broth with the medicated comma-bacilli I have obtained normal growths of them. Other media known to have a noxious effect on bacteria in general, have the same effect on the choleraic comma-bacilli.

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