The Bacteriological Examination of Water-Supplies

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Then the calculated amount of alkali, less this deduction, is added, and a + ro per cent, reaction is obtained. In other words, alkali is added insufficient to quite neutralize the medium (phenolphthalein neutral point), and the addition of 1*0 per cent, of £ alkali would still be required to make it completely neutral.
As an example of an actual standardization, the follow- ing is given : Two separate 10 c.c. of a i litre of nutrient broth each required the addition of 1*3 c.c. -^ sodium hydra
...te solution to make them neutral to phenolphthalein. Therefore, 980 c.c. (i.e,j 1,000 - 20 c.c.) will require ( l -^ x 980^ = \ 10 / 127*4 c.c. tu sodium hydrate solution. This is equal to 1274 (^>~p)cc of g alkali.
One c.c. of £ acid is deducted for each 100 c.c, or 10 c.c.
for the litre. Therefore, 274 (1274-10) c.c. £ alkali is added to the medium, and a + i'o reaction is obtained.
If the medium is already alkaline to phenolphthalein, the sample removed for titration should be made acid by the addition of a definite and accurately pipetted amount of ^j H 2 S0 4 .


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