The Chemical Nature of the Antigenic Substances in Bacillus Coli

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"ffoicneqej ircf b^ct scf ^oon e*! orfj 10! el :f> lo ei^eb tfneiollljfl fitirrse lo r. Qcib rroT L Oi1^ i^dle fcne ^e^i^fl^ ss*! snivrolfcl xlrf t -sodunlm 03 elcfj v : 17 Table #23. Pat Antigen and Anti-coli Serum.
Dilution of Serum Result 1:25 + 1:50 + 1:75 + 1:100 1:125 1:150 1:175 Table #24. Pat Antigen and Antiprotein Serum Dilution of Serum Result 1:25 1:50 1:75 1:100 1:125 1:150 1:175 Table #25. Fat Antigen and Normal Serum Dilution of Serum Result 1:25 1:50 1:75 1 : 100 1:125 1:150 1:1
...75 + = Complete precipitation * = Partial " - = No " The results are very indicative of a specific pre- cipitatin reaction, in which the fats play the most important part. Too much weight, however, should not be attached to these reactions of precipitins because in some cases (preliminary titrations) spontaneous reaction occurred. Such reactions also frequently occurred after the fifteen minute incubation period. This was probably due to the unstable dispersoid phase of the antigen, so that it was very susceptible to the reactions of the various sera.

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