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The Effect of Large Applications of Commercial Fertilizers On Carnations

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^^"^^ Andre 1 and also Dixon and Atkins'^ have shown that successive por- tions of sap expressed from unfrozen tissue become more concentrated, while the latter have shown that the sap from frozen tissue always has a lower freezing point than that from unfrozen, and that successive portions gave nearly identical lowerings, leading to the conclusion that sap so expressed is representative of that originally within the tissue.
13 the laboratory. After removal of the foliage from the stems, they w
...ere broken at the nodes and placed in hard glass test tubes (25 mm. X 150 mm. ), stoppered with rubber stoppers and sealed with oil paper and rub- ber bands. Freezing was produced by the use of the ice and salt bath, * giving a temperature of —15° or lower and allowing the tubes to remain in the refrigerator overnight. The tubes were then removed from the bath and after the walls had been cleaned with distilled water and wiped dry, the portions of shoots were removed, thawed gradually, and the sap ex- pressed by pressure from the screw of a tincture press set perpendicular to the wall upon two pieces of Vs inch plate glass.

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