The Proteins of the Wheat Kernel

Cover The Proteins of the Wheat Kernel
The Proteins of the Wheat Kernel
Thomas B Thomas Burr Osborne
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Weight.
I II Ill IV o 93 loo I20 i6o 75-76 mm.
12.0 12.0 0.8 0.8 Grams.
28.18 47.03 64.68 40.00 83 100 120 200 mm, 12.0 12.0 0.8 0.8 Grams.
20.13 3681 62.70 3300 179.89 152.64 Fraction I.
DisHUation A. — This was saponified at once by evaporation with con- centrated hydrochloric acid on a water-bath, the residue taken up in alcohol, the solution saturated with dry hydrochloric acid gas, and a crystal of glycocoU ester hydrochloride added. After prolonged standing on ice, no separation occurred.
... The solution was then evaporated on the water- bath with concentrated hydrochloric add, the latter removed with lead oxide and the lead with hydrogen sulphide. The amino-acids were subjected to fractional crystallization.
DisHUaHan B vrzs treated in substantially the same way, but although several attempts were made to isolate the hydrochloride of glycocoU ester, none was found. By systematic fractionation there were obtained from fraction I of the two distillations, A and B, 6.68 grams alanine and 0.86 gram leucine.


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