A Comparative And Experimental Study of Bacilli Producing Red Pigment

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Ruber miquel and B. Plym. II, the orange tone still held for the series on this medium, i. E. , the alkali produced by the bacteria was not neutralized by acid formed from the sugar. It would seem that since B. Ruber balticus, B. Plymouthensis I, and B. Miniaceus ferment lactose with gas production, these cul- tures would show a decrease of the orange tone.
Although the relative results arrived at on the above media are of some value, their absolute value is lessened because of the unknown fact
...ors present in agar and peptone. Agar itself contains a certain amount of carbohydrate, galactose (Bauer, Jour. Prakt. Chemie, B. XXX. P. 367), which is probably assimilable by the organisms. A further reduction of the medium, by leaving out the peptone, resulted in very feeble white growth in five days. Three cultures only showed a trace of pigment, B. Ruber indicus II, B. Rutilus, and B. Prodigiosus VII. The last named showed a green iridescence on the thin pink layer.
In the endeavor to do away with agar and still have a solid medium for pigment production, the following mixtures were employed, and gave interesting comparative results.


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