Abstract of Minutes, 1889-1895
Abstract of Minutes, 1889-1895
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The test is performed as follows: to a test-tube add o.i gram of phenylhydrazine hydrochloride, 0.5 cubic centimeter of a 50% solution of soditun acetate, and about one cubic centimeter of tuine. Heat over free flame, and then in boiling water for 30 minutes. At the end of that time, allow it to cool, and examine a drop of the mixture under the microscope. Various sugars form definitely characteristic crystals called phenylosazones such as phenyl- glucosazone, phenylpentazone, etc. In case suga...r is not re- sponsible for the reduction, the osazone will not be formed.
Glucose forms fine, straight needles lying in sheaf formation.
Pentose generally forms very thin wavy crystals which may or may not be in sheaves. Whenever there is doubt as to whether an osazone be glucose or pentose we always perform a Bial's test. If a grass-green color appears in the Bial's test, extract with amyl alcohol and look for an absorption band in the red near the yellow in the spectroscope. In all our experience with the phenylhydrazine test, we have rarely found it to form phenylglucosazone luiless glucose was present in 0.25% or more, and these exceptions occurred in low gravity tuines, to which significance could be attached.
What to read after Abstract of Minutes, 1889-1895?
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To quickly assess the difficulty of the text, read a short excerpt:
The test is performed as follows: to a test-tube add o.i gram of phenylhydrazine hydrochloride, 0.5 cubic centimeter of a 50% solution of soditun acetate, and about one cubic centimeter of tuine. Heat over free flame, and then in boiling water for 30 minutes. At the end of that time, allow it to cool, and examine a drop of the mixture under the microscope. Various sugars form definitely characteristic crystals called phenylosazones such as phenyl- glucosazone, phenylpentazone, etc. In case suga...r is not re- sponsible for the reduction, the osazone will not be formed.
Glucose forms fine, straight needles lying in sheaf formation.
Pentose generally forms very thin wavy crystals which may or may not be in sheaves. Whenever there is doubt as to whether an osazone be glucose or pentose we always perform a Bial's test. If a grass-green color appears in the Bial's test, extract with amyl alcohol and look for an absorption band in the red near the yellow in the spectroscope. In all our experience with the phenylhydrazine test, we have rarely found it to form phenylglucosazone luiless glucose was present in 0.25% or more, and these exceptions occurred in low gravity tuines, to which significance could be attached.
What to read after Abstract of Minutes, 1889-1895?
You can find similar books in the "Read Also" column, or choose other free books by Association of Life Insurance Medical Directors of America to read onlineMoreLess
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