Biochemical Studies of Mercaptan ..
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proteus vulgaris and o.i gram of solid cystin; tube 2, 9 cc. of the peptone culture and i cc. of a 0.05 per cent, suspension of cystin in water; tube 3, 8 cc. of the culture and 2 cc. of the cystin; suspension tube 4, 6 cc. of the culture and 4 cc. of the cystin suspension. All the fer- mentation tubes were incubated at 38° C. In 48 hours there was gas formation in all the tubes except number i.
In 72 hours this also showed the presence of gas in the long arm of the tube. The gas in tubes 2, 3,... and 4 smelt strongly of mercaptan. Tube number i smelt of hydrogen sulfide rather than of mercaptan.
This experiment was modified. A flask was connected by means of glass tubes so that any escaping gas would be collected in a solution of isatin in sulfuric acid. The flask was filled with 50 cc. of the peptone culture of the proteus vulgaris and o.i gram of cystin. At the end of 48 hours in the incubator the isatin-sulfuric acid was tinged with green, and at the end of 72 hours was entirely green.
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You can find similar books in the "Read Also" column, or choose other free books by Goodridge, Frederic Grosvenor, B. 1874 to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
proteus vulgaris and o.i gram of solid cystin; tube 2, 9 cc. of the peptone culture and i cc. of a 0.05 per cent, suspension of cystin in water; tube 3, 8 cc. of the culture and 2 cc. of the cystin; suspension tube 4, 6 cc. of the culture and 4 cc. of the cystin suspension. All the fer- mentation tubes were incubated at 38° C. In 48 hours there was gas formation in all the tubes except number i.
In 72 hours this also showed the presence of gas in the long arm of the tube. The gas in tubes 2, 3,... and 4 smelt strongly of mercaptan. Tube number i smelt of hydrogen sulfide rather than of mercaptan.
This experiment was modified. A flask was connected by means of glass tubes so that any escaping gas would be collected in a solution of isatin in sulfuric acid. The flask was filled with 50 cc. of the peptone culture of the proteus vulgaris and o.i gram of cystin. At the end of 48 hours in the incubator the isatin-sulfuric acid was tinged with green, and at the end of 72 hours was entirely green.
What to read after Biochemical Studies of Mercaptan ..?
You can find similar books in the "Read Also" column, or choose other free books by Goodridge, Frederic Grosvenor, B. 1874 to read onlineMoreLess
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