Examination of Water, Chemical And Bacteriological

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Strain through a cloth and make up to one litre with distilled water. Add Gelatine (best French) 100 grammes Peptone (Witte's); 10 Common salt 5 " Heat in a double-walled " oatmeal-boiler " at a tem- perature between 35° and 40° C. until all is dissolved.
Add the white of one ^z^, previously shaken up with BACTERIOLOGICAL EXAMINATION. 97 about its own bulk of water, and stir thoroughly.
Heat as rapidly as possible to boiling, stirring occa- sionally. Cover the mixture and keep the water in the
...outer vessel boiling during fifty minutes.
Filter, with the use of the hot-water funnel. Place 5 c.c. of the filtered medium in a porcelain dish ; dilute the same with 45 c.c. water ; titrate with N/20 NaHO solution, as in the case of the prep- aration of " bouillon," and from the data so ob- tained calculate what addition of normal HCl should be made to the main bulk in order to carry its reac- tion to the desired point, namely, + 15.
Pour the finished jelly into " Miquel flasks " (20 c.c. in each) or test-tubes (10 c.c.


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