Onion Smudge

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— Studies were made on conidia as they occur (i) in masses on the fruiting body on the host, where they are embedded in the mucilaginous material which surrounds them, (2) in similar masses on potato agar, and (3) in water suspension, where the spores are sepa- rated from one another, approximating to some extent conditions as Onion Stmidge 699 they occur in nature when spores are disseminated by meteoric water.
Diseased onions bearing spore masses were brought in and allowed to dry out gradual
...ly in the laboratory, and the viability of the spores was tested from time to time. Ordinarily a large percentage lost their vitality within 2 weeks, but in some cases good germination occurred after 7 weeks. A small percentage of conidia from spore masses pro- duced on potato agar and exposed to similar conditions germinated after 4 months. Spores in water suspension allowed to dry out on glass slides were very sensitive to desiccation, little or no germination occurring after 24 hours. It is evident, then, that the conidia are sensitive to desiccation except when they remain in waxy masses on the host, in which condition a small percentage will remain viable through extended unfavorable periods.

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