Practical Exercises in Comparative Physiology And Urine Analysis

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88. Dilute the .saliva with an equal volume of distilled water. Label four test tubes, A, B, C, and D. Into A place some .saliva, boil it and add some starch mucilage. In B and C place starch mucilage and saliva, to B add a few drops of hydrochloric acid and to C some caustic potash.
To D add merely the saliva to the starch mixture.
Place all four in a water bath not exceeding 40° C, and after a time test a small portion of them for .sugar with Fehling's .solution. Reserve a small amount of D.
...Why is no sugar formed in A? In B and C a strong acid and alkali arrest the action of ptyalin. Neutralize a portion of B and C and test again. Is there any result? In D the starch has been converted by the ptyalin into a reducing sugar.
89. Test a portion of D with iodine solution. The ab- sence of any blue color indicates that the starch has disap- peared, having been converted into a reducing sugar — mal- tose. Also test the remainder of A, B and C with the iodine .solution.
90. Test another portion of D withphenyl-hydraziu, (53) crystals of phenyl-maltosazone should develop.


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