Use And Care of the Microscope Extracts From Manipulation of the Microscope

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To make immersion contact between condenser and slide place a drop of oil on the top of condenser, drop the slide upon the stage, first throwing the clips to one side.
With immersion objectives the proper focusing of the condenser becomes a matter of nice distinction to obtain best results and can only be reliably accomplished by considerable practice and experience. To obtain best position : Use a 2-j objective ; focus upon the object ; adjust condenser until image of window-sash or flame is i
...n the same plane with the object.
Complete Substage. Top view, with condenser and lower iris diaphragm swung out of optical axis.
Relation of Aperture of Condenser to Objective. In the study of Bacteria and other micro- organisms, the objectives used being of wide aperture, it is sought to have them stand out boldly in a bright field. This is accomplished by bringing the diaphragm to its full aperture. On all other objects, however, too much illumination decidedly injures definition by obliterating detail.


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